2DGE-coomassie brilliant blue staining used to differentiate pasteurized milk from reconstituted milk

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Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

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ژورنال

عنوان ژورنال: Health

سال: 2009

ISSN: 1949-4998,1949-5005

DOI: 10.4236/health.2009.13024